Me: Everytime I got time on the confocal

Inspired by ohio valley style pizza

An 18 year old can decide their own fate here, hopefully they don’t have a kid of their own yet

I can think of 20-30 more interesting stories and characters to make a TV show on from the DC universe

I’ve been buying used 12tb HGST from Amazon for $80.

Wait until they learn the Spanish word for black, or worse Latin.

Im the one that was paying for Netflix for my family, but the password crack down motivated me to learn how to build a server and go full arrs. They had a good thing going, but now that $26 a month will be used to buy hdds.

Lol yeh but it’s partially funded by money saved from canceled subscriptions.

I’m a noob, I started 2 months ago with immich and tailscale. Now I have an unraid server, it’s a slippery slope.

Pretty good description. Few extra points. -you don’t need to don’t need to do PCR to have enough Nucleic acids to visualize on the gel. Often you use gels to visualize things like purified plasmids. You can also digest your plasmids with enzymes to confirm genomic insert. -these type of gels have many uses outside of direct comparisons, for example you can use the gel purity DNA of a particular size by cutting out the band, melting the gel and using a silica column to pull the DNA out of the melted gel slice. This is super common when you do molecular cloning.

For what is possibly wrong with the top gel.

• the wavy line in some bands indicates uneven current, this can be due to sample composition before loading the gel, like having cellular debris along with your DNA or a mismatch between the buffer used to make the gel and the running buffer.

-the blurred bands can be a lot of things. It might just be inherited to the sample type, for example raw genomic DNA will look like this because you’ll have enzyme that degrade DNA, and a lot of mid replication chromosomes resulting in fragments existing across a spectrum of sizes equally. Additionally the it could also be user error with overloaded well or not enough loading buffer in the sample so it’s not pushed in to gel evenly, or it was run to fast.

Overall the top gel just looks like crap and the bottom gel looks great, but depending on what was run you really don’t know what the gel should look like, if I was testing endonuclease activity the top gel would probably be what I was hoping to get. If was doing PCR on single gene, the bottom one is better.

That’s probably more of a reason to get her vaccinated. Those topical flea and tick meds are rough. We got our cat vaccinated even though she was 100% indoor because she used to get too sick with topicals and liked to cuddle the dog.

Poor thing, I’m glad he has a loving family now. I once found a golden retriever wandering the forest while hiking in Indiana. She had so many ticks on her we had to take her to an emergency vet (since it was Sunday) to get them off. Luckly she was microchipped and we found her family who has been looking for her for over a week at the time.

If only there was a vaccine for Lyme disease so the poor pupper wouldn’t have had to suffer in the first place